A new regulator of pathogenicity (bvlR) is required for full virulence and tight microcolony formation in Pseudomonas aeruginosa.
Identifieur interne : 003C45 ( Main/Exploration ); précédent : 003C44; suivant : 003C46A new regulator of pathogenicity (bvlR) is required for full virulence and tight microcolony formation in Pseudomonas aeruginosa.
Auteurs : Ronan R. Mccarthy [Irlande (pays)] ; Marlies J. Mooij [Irlande (pays)] ; F Jerry Reen [Irlande (pays)] ; Olivier Lesouhaitier [France] ; Fergal O'Gara [Irlande (pays)]Source :
- Microbiology (Reading, England) [ 1465-2080 ] ; 2014.
Descripteurs français
- KwdFr :
- ADP ribose transferases (métabolisme), Adhérence bactérienne, Animaux, Biofilms (croissance et développement), Caenorhabditis elegans (microbiologie), Exotoxines (métabolisme), Facteurs de transcription (génétique), Facteurs de transcription (isolement et purification), Facteurs de transcription (métabolisme), Facteurs de virulence (génétique), Facteurs de virulence (isolement et purification), Facteurs de virulence (métabolisme), Homéostasie, Humains, Infections à Pseudomonas (microbiologie), Protéines bactériennes (génétique), Protéines bactériennes (isolement et purification), Protéines bactériennes (métabolisme), Protéines de répression (génétique), Protéines de répression (isolement et purification), Protéines de répression (métabolisme), Pseudomonas aeruginosa (génétique), Pseudomonas aeruginosa (pathogénicité), Pseudomonas aeruginosa (physiologie), Régulation de l'expression des gènes bactériens, Systèmes bactériens de sécrétion, Toxines bactériennes (métabolisme), Virulence.
- MESH :
- croissance et développement : Biofilms.
- génétique : Facteurs de transcription, Facteurs de virulence, Protéines bactériennes, Protéines de répression, Pseudomonas aeruginosa.
- isolement et purification : Facteurs de transcription, Facteurs de virulence, Protéines bactériennes, Protéines de répression.
- microbiologie : Caenorhabditis elegans, Infections à Pseudomonas.
- métabolisme : ADP ribose transferases, Exotoxines, Facteurs de transcription, Facteurs de virulence, Protéines bactériennes, Protéines de répression, Toxines bactériennes.
- pathogénicité : Pseudomonas aeruginosa.
- physiologie : Pseudomonas aeruginosa.
- Adhérence bactérienne, Animaux, Homéostasie, Humains, Régulation de l'expression des gènes bactériens, Systèmes bactériens de sécrétion, Virulence.
English descriptors
- KwdEn :
- ADP Ribose Transferases (metabolism), Animals, Bacterial Adhesion, Bacterial Proteins (genetics), Bacterial Proteins (isolation & purification), Bacterial Proteins (metabolism), Bacterial Secretion Systems, Bacterial Toxins (metabolism), Biofilms (growth & development), Caenorhabditis elegans (microbiology), Exotoxins (metabolism), Gene Expression Regulation, Bacterial, Homeostasis, Humans, Pseudomonas Infections (microbiology), Pseudomonas aeruginosa (genetics), Pseudomonas aeruginosa (pathogenicity), Pseudomonas aeruginosa (physiology), Repressor Proteins (genetics), Repressor Proteins (isolation & purification), Repressor Proteins (metabolism), Transcription Factors (genetics), Transcription Factors (isolation & purification), Transcription Factors (metabolism), Virulence, Virulence Factors (genetics), Virulence Factors (isolation & purification), Virulence Factors (metabolism).
- MESH :
- chemical , genetics : Bacterial Proteins, Repressor Proteins, Transcription Factors, Virulence Factors.
- chemical , isolation & purification : Bacterial Proteins, Repressor Proteins, Transcription Factors, Virulence Factors.
- chemical , metabolism : ADP Ribose Transferases, Bacterial Proteins, Bacterial Toxins, Exotoxins, Repressor Proteins, Transcription Factors, Virulence Factors.
- genetics : Pseudomonas aeruginosa.
- growth & development : Biofilms.
- microbiology : Caenorhabditis elegans, Pseudomonas Infections.
- pathogenicity : Pseudomonas aeruginosa.
- physiology : Pseudomonas aeruginosa.
- Animals, Bacterial Adhesion, Bacterial Secretion Systems, Gene Expression Regulation, Bacterial, Homeostasis, Humans, Virulence.
Abstract
LysR-type transcriptional regulators (LTTRs) are the most common family of transcriptional regulators found in the opportunistic pathogen Pseudomonas aeruginosa. They are known to regulate a wide variety of virulence determinants and have emerged recently as positive global regulators of pathogenicity in a broad spectrum of important bacterial pathogens. However, in spite of their key role in modulating expression of key virulence determinants underpinning pathogenic traits associated with the process of infection, surprisingly few are found to be transcriptionally altered by contact with host cells. BvlR (PA14_26880) an LTTR of previously unknown function, has been shown to be induced in response to host cell contact, and was therefore investigated for its potential role in virulence. BvlR expression was found to play a pivotal role in the regulation of acute virulence determinants such as type III secretion system and exotoxin A production. BvlR also played a key role in P. aeruginosa pathogenicity within the Caenorhabditis elegans acute model of infection. Loss of BvlR led to an inability to form tight microcolonies, a key step in biofilm formation in the cystic fibrosis lung, although surface attachment was increased. Unusually for LTTRs, BvlR was shown to exert its influence through the transcriptional repression of many genes, including the virulence-associated cupA and alg genes. This highlights the importance of BvlR as a new virulence regulator in P. aeruginosa with a central role in modulating key events in the pathogen-host interactome.
DOI: 10.1099/mic.0.075291-0
PubMed: 24829363
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003618
- to stream PubMed, to step Curation: 003505
- to stream PubMed, to step Checkpoint: 003505
- to stream Ncbi, to step Merge: 001A36
- to stream Ncbi, to step Curation: 001A36
- to stream Ncbi, to step Checkpoint: 001A36
- to stream Main, to step Merge: 003C96
- to stream Main, to step Curation: 003C45
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A new regulator of pathogenicity (bvlR) is required for full virulence and tight microcolony formation in Pseudomonas aeruginosa.</title><author><name sortKey="Mccarthy, Ronan R" sort="Mccarthy, Ronan R" uniqKey="Mccarthy R" first="Ronan R" last="Mccarthy">Ronan R. Mccarthy</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Mooij, Marlies J" sort="Mooij, Marlies J" uniqKey="Mooij M" first="Marlies J" last="Mooij">Marlies J. Mooij</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland f.ogara@ucc.ie marlies.mooij@mumc.nl.</nlm:affiliation><country wicri:rule="url">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Reen, F Jerry" sort="Reen, F Jerry" uniqKey="Reen F" first="F Jerry" last="Reen">F Jerry Reen</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Lesouhaitier, Olivier" sort="Lesouhaitier, Olivier" uniqKey="Lesouhaitier O" first="Olivier" last="Lesouhaitier">Olivier Lesouhaitier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA4312, University of Rouen, 55 rue Saint Germain, 27000 Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA4312, University of Rouen, 55 rue Saint Germain, 27000 Evreux</wicri:regionArea><wicri:noRegion>27000 Evreux</wicri:noRegion><wicri:noRegion>27000 Evreux</wicri:noRegion></affiliation></author><author><name sortKey="O Gara, Fergal" sort="O Gara, Fergal" uniqKey="O Gara F" first="Fergal" last="O'Gara">Fergal O'Gara</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland Curtin University, School of Biomedical Sciences, Perth, WA, Australia f.ogara@ucc.ie marlies.mooij@mumc.nl.</nlm:affiliation><country wicri:rule="url">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland Curtin University, School of Biomedical Sciences, Perth, WA</wicri:regionArea><wicri:noRegion>WA</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24829363</idno><idno type="pmid">24829363</idno><idno type="doi">10.1099/mic.0.075291-0</idno><idno type="wicri:Area/PubMed/Corpus">003618</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003618</idno><idno type="wicri:Area/PubMed/Curation">003505</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003505</idno><idno type="wicri:Area/PubMed/Checkpoint">003505</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003505</idno><idno type="wicri:Area/Ncbi/Merge">001A36</idno><idno type="wicri:Area/Ncbi/Curation">001A36</idno><idno type="wicri:Area/Ncbi/Checkpoint">001A36</idno><idno type="wicri:Area/Main/Merge">003C96</idno><idno type="wicri:Area/Main/Curation">003C45</idno><idno type="wicri:Area/Main/Exploration">003C45</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A new regulator of pathogenicity (bvlR) is required for full virulence and tight microcolony formation in Pseudomonas aeruginosa.</title><author><name sortKey="Mccarthy, Ronan R" sort="Mccarthy, Ronan R" uniqKey="Mccarthy R" first="Ronan R" last="Mccarthy">Ronan R. Mccarthy</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Mooij, Marlies J" sort="Mooij, Marlies J" uniqKey="Mooij M" first="Marlies J" last="Mooij">Marlies J. Mooij</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland f.ogara@ucc.ie marlies.mooij@mumc.nl.</nlm:affiliation><country wicri:rule="url">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Reen, F Jerry" sort="Reen, F Jerry" uniqKey="Reen F" first="F Jerry" last="Reen">F Jerry Reen</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Lesouhaitier, Olivier" sort="Lesouhaitier, Olivier" uniqKey="Lesouhaitier O" first="Olivier" last="Lesouhaitier">Olivier Lesouhaitier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA4312, University of Rouen, 55 rue Saint Germain, 27000 Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA4312, University of Rouen, 55 rue Saint Germain, 27000 Evreux</wicri:regionArea><wicri:noRegion>27000 Evreux</wicri:noRegion><wicri:noRegion>27000 Evreux</wicri:noRegion></affiliation></author><author><name sortKey="O Gara, Fergal" sort="O Gara, Fergal" uniqKey="O Gara F" first="Fergal" last="O'Gara">Fergal O'Gara</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland Curtin University, School of Biomedical Sciences, Perth, WA, Australia f.ogara@ucc.ie marlies.mooij@mumc.nl.</nlm:affiliation><country wicri:rule="url">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland Curtin University, School of Biomedical Sciences, Perth, WA</wicri:regionArea><wicri:noRegion>WA</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbiology (Reading, England)</title><idno type="eISSN">1465-2080</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ADP Ribose Transferases (metabolism)</term><term>Animals</term><term>Bacterial Adhesion</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (isolation & purification)</term><term>Bacterial Proteins (metabolism)</term><term>Bacterial Secretion Systems</term><term>Bacterial Toxins (metabolism)</term><term>Biofilms (growth & development)</term><term>Caenorhabditis elegans (microbiology)</term><term>Exotoxins (metabolism)</term><term>Gene Expression Regulation, Bacterial</term><term>Homeostasis</term><term>Humans</term><term>Pseudomonas Infections (microbiology)</term><term>Pseudomonas aeruginosa (genetics)</term><term>Pseudomonas aeruginosa (pathogenicity)</term><term>Pseudomonas aeruginosa (physiology)</term><term>Repressor Proteins (genetics)</term><term>Repressor Proteins (isolation & purification)</term><term>Repressor Proteins (metabolism)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (isolation & purification)</term><term>Transcription Factors (metabolism)</term><term>Virulence</term><term>Virulence Factors (genetics)</term><term>Virulence Factors (isolation & purification)</term><term>Virulence Factors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADP ribose transferases (métabolisme)</term><term>Adhérence bactérienne</term><term>Animaux</term><term>Biofilms (croissance et développement)</term><term>Caenorhabditis elegans (microbiologie)</term><term>Exotoxines (métabolisme)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (isolement et purification)</term><term>Facteurs de transcription (métabolisme)</term><term>Facteurs de virulence (génétique)</term><term>Facteurs de virulence (isolement et purification)</term><term>Facteurs de virulence (métabolisme)</term><term>Homéostasie</term><term>Humains</term><term>Infections à Pseudomonas (microbiologie)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (isolement et purification)</term><term>Protéines bactériennes (métabolisme)</term><term>Protéines de répression (génétique)</term><term>Protéines de répression (isolement et purification)</term><term>Protéines de répression (métabolisme)</term><term>Pseudomonas aeruginosa (génétique)</term><term>Pseudomonas aeruginosa (pathogénicité)</term><term>Pseudomonas aeruginosa (physiologie)</term><term>Régulation de l'expression des gènes bactériens</term><term>Systèmes bactériens de sécrétion</term><term>Toxines bactériennes (métabolisme)</term><term>Virulence</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Repressor Proteins</term><term>Transcription Factors</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Bacterial Proteins</term><term>Repressor Proteins</term><term>Transcription Factors</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>ADP Ribose Transferases</term><term>Bacterial Proteins</term><term>Bacterial Toxins</term><term>Exotoxins</term><term>Repressor Proteins</term><term>Transcription Factors</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Biofilms</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pseudomonas aeruginosa</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Biofilms</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term><term>Facteurs de virulence</term><term>Protéines bactériennes</term><term>Protéines de répression</term><term>Pseudomonas aeruginosa</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Facteurs de transcription</term><term>Facteurs de virulence</term><term>Protéines bactériennes</term><term>Protéines de répression</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Caenorhabditis elegans</term><term>Infections à Pseudomonas</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Caenorhabditis elegans</term><term>Pseudomonas Infections</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADP ribose transferases</term><term>Exotoxines</term><term>Facteurs de transcription</term><term>Facteurs de virulence</term><term>Protéines bactériennes</term><term>Protéines de répression</term><term>Toxines bactériennes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Pseudomonas aeruginosa</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Pseudomonas aeruginosa</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Pseudomonas aeruginosa</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Pseudomonas aeruginosa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bacterial Adhesion</term><term>Bacterial Secretion Systems</term><term>Gene Expression Regulation, Bacterial</term><term>Homeostasis</term><term>Humans</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adhérence bactérienne</term><term>Animaux</term><term>Homéostasie</term><term>Humains</term><term>Régulation de l'expression des gènes bactériens</term><term>Systèmes bactériens de sécrétion</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">LysR-type transcriptional regulators (LTTRs) are the most common family of transcriptional regulators found in the opportunistic pathogen Pseudomonas aeruginosa. They are known to regulate a wide variety of virulence determinants and have emerged recently as positive global regulators of pathogenicity in a broad spectrum of important bacterial pathogens. However, in spite of their key role in modulating expression of key virulence determinants underpinning pathogenic traits associated with the process of infection, surprisingly few are found to be transcriptionally altered by contact with host cells. BvlR (PA14_26880) an LTTR of previously unknown function, has been shown to be induced in response to host cell contact, and was therefore investigated for its potential role in virulence. BvlR expression was found to play a pivotal role in the regulation of acute virulence determinants such as type III secretion system and exotoxin A production. BvlR also played a key role in P. aeruginosa pathogenicity within the Caenorhabditis elegans acute model of infection. Loss of BvlR led to an inability to form tight microcolonies, a key step in biofilm formation in the cystic fibrosis lung, although surface attachment was increased. Unusually for LTTRs, BvlR was shown to exert its influence through the transcriptional repression of many genes, including the virulence-associated cupA and alg genes. This highlights the importance of BvlR as a new virulence regulator in P. aeruginosa with a central role in modulating key events in the pathogen-host interactome.</div></front></TEI><affiliations><list><country><li>France</li><li>Irlande (pays)</li></country></list><tree><country name="Irlande (pays)"><noRegion><name sortKey="Mccarthy, Ronan R" sort="Mccarthy, Ronan R" uniqKey="Mccarthy R" first="Ronan R" last="Mccarthy">Ronan R. Mccarthy</name></noRegion><name sortKey="Mooij, Marlies J" sort="Mooij, Marlies J" uniqKey="Mooij M" first="Marlies J" last="Mooij">Marlies J. Mooij</name><name sortKey="O Gara, Fergal" sort="O Gara, Fergal" uniqKey="O Gara F" first="Fergal" last="O'Gara">Fergal O'Gara</name><name sortKey="Reen, F Jerry" sort="Reen, F Jerry" uniqKey="Reen F" first="F Jerry" last="Reen">F Jerry Reen</name></country><country name="France"><noRegion><name sortKey="Lesouhaitier, Olivier" sort="Lesouhaitier, Olivier" uniqKey="Lesouhaitier O" first="Olivier" last="Lesouhaitier">Olivier Lesouhaitier</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003C45 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003C45 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24829363
|texte= A new regulator of pathogenicity (bvlR) is required for full virulence and tight microcolony formation in Pseudomonas aeruginosa.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24829363" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |